Mitochondrial Medicine : Volume I, Probing Mitochondrial Function - Weissig, Volkmar; Edeas, Marvin; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Preparation of 'Functional' Mitochondria: A Challenging Business -- Isolation and Quality Control of Functional Mitochondria -- Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells -- Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels -- Optical Microwell Arrays for Large Scale Studies of Single Mitochondria Metabolic Responses -- Deep Resequencing of Mitochondrial DNA -- Single Cell Analysis of Mitochondrial DNA -- A High-Throughput Next-Generation Sequencing Assay for the Mitochondrial Genome -- Rapid Mitochondrial DNA Isolation Method for Direct Sequencing -- Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR Based Method -- Mitochondria-Targeted RNA Import -- Accurate Measurement of Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR -- mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution -- Simultaneous Quantification of Mitochondrial ATP and ROS Production -- Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine -- Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid -- Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells -- Novel Methods for Measuring the Mitochondrial Membrane Potential -- High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes -- In Vivo Assessment of Mitochondrial Oxygen Consumption -- Imaging Mitochondrial Hydrogen Peroxide in Living Cells -- Simultaneous High-Resolution Measurement of Mitochondrial Respiration and Hydrogen Peroxide Production -- Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells -- Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry -- Assessing the Bioenergetic Profile of Human Pluripotent Stem Cells -- Integrative Methods for Studying Cardiac Energetics -- Computer-Based Prediction of Mitochondria-Targeting Peptides -- Prediction of Mitochondrial Protein Function by Comparative Physiology and Phylogenetic Profiling -- Assessment of Posttranslational Modification of Mitochondrial Proteins -- Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway -- High-Resolution Melting Analysis for Identifying Sequence Variations in Nuclear Genes for Assembly Factors and Structural Subunits of Cytochrome C Oxidase -- Heterologous Inferential Analysis (HIA) as a Method to Understand the Role of Mitochondrial rRNA Mutations in Pathogenesis -- Analysis of Mitochondrial Dysfunction During Cell Death -- The Use of FLIM-FRET for the Detection of Mitochondria-Associated Protein Interactions -- Assessment of Mitochondrial Ca2+ Uptake -- Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS -- Characterization of Mitochondrial Populations During Stem Cell Differentiation -- An Ex Vivo Model for Studying Mitochondrial Trafficking in Neurons.
Keywords: Molecular biology; Cell culture; Molecular Medicine; Cell Culture