Innate Immune Activation : Methods and Protocols - De Nardo, Dominic; De Nardo, Christine M.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Emerging concepts in innate immunity -- Bioinformatic Assessment of macrophage activation by the innate immune system -- Generation of Genetic Knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system -- Modeling primary human monocytes with the trans-differentiation cell line BLaER1 -- Measurement of NF-κB activation in TLR-activated macrophages -- Biochemical isolation of the myddosome from murine macrophages -- Generation of innate immune reporter cell lines using retroviral transduction -- Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER) -- Inflammatory caspases: Activation and cleavage of Gasdermin-D in vitro and during Pyroptosis -- Methods for detecting ASC and ASC speck formation -- Measuring innate immune responses to bacterial viability -- Methods to study cell swelling-induced inflammasome activation -- Detecting release of bacterial dsDNA into the host cytosol using fluorescence microscopy -- Quantitative proteomics of secreted proteins -- Simultaneous detection of cellular viability and interleukin-1β secretion from single cells by ELISpot -- Detection and quantification of MAV aggregation via confocal imaging. .
Keywords: Immunology; Immunology