Helicases : Methods and Protocols - Abdelhaleem, Mohamed M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Helicases: An Overview -- Fluorescent Biosensors to Investigate Helicase Activity -- Single-Molecule FRET Analysis of Helicase Functions -- Kinetics of Motor Protein Translocation on Single-Stranded DNA -- Experimental and Computational Analysis of DNA Unwinding and Polymerization Kinetics -- Protein Displacement by Helicases -- In Vivo Investigation of Protein-Protein Interactions for Helicases Using Tandem Affinity Purification -- Mapping Genomic Targets of DNA Helicases by Chromatin Immunoprecipitation in Saccharomyces cerevisiae -- Methods to Study How Replication Fork Helicases Unwind DNA -- Simple Enzymatic Assays for the In Vitro Motor Activity of Transcription Termination Factor Rho from Escherichia coli -- Single-Molecule Studies of RecBCD -- Mutational Analysis of Bloom Helicase -- In Vivo Analysis of Drosophila BLM Helicase Function During DNA Double-Strand Gap Repair -- Purification and Characterization of RecQ Helicases of Plants -- Fluorometric Assay of Hepatitis C Virus NS3 Helicase Activity -- A Method to Simultaneously Monitor Hepatitis C Virus NS3 Helicase and Protease Activities -- Computer Modeling of Helicases Using Elastic Network Model -- Duplex Unwinding with DEAD-Box Proteins -- Analysis of the RNA Helicase p68 (Ddx5) as a Transcriptional Regulator -- A Method to Study the Role of DDX3 RNA Helicase in HIV-1 -- Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A) -- Regulation of Inter- and Intramolecular Interaction of RNA, DNA, and Proteins by MLE -- Biochemical Characterization of Human Upf1 Helicase -- Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome -- Characterization of the Helicase Activity and Anti-telomerase Properties of Yeast Pif1p In Vitro -- A Method to Confer Salinity Stress Tolerance to Plants by Helicase Overexpression -- A Method to Inhibit the Growth of Plasmodium falciparum by Double-Stranded RNA-Mediated Gene Silencing of Helicases.
Keywords: Biochemistry; Proteins .; Enzymology; Biochemistry, general; Protein Science; Enzymology