Heterologous Gene Expression in E.coli : Methods and Protocols - Evans, Jr., Thomas C.; Xu, Ming-Qun.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Adjustment of Codon Usage Frequencies by Codon Harmonization Improves Protein Expression and Folding -- SUMO Fusion Technology for Enhanced Protein Expression and Purification in Prokaryotes and Eukaryotes -- Molecular and Chemical Chaperones for Improving the Yields of Soluble Recombinant Proteins -- Genetic Selection of Solubility-Enhanced Proteins Using the Twin-Arginine Translocation System -- Protein Folding Liquid Chromatography -- Site-Specific Protein Labeling by Intein-Mediated Protein Ligation -- Efficient Expression of Human Aromatase (CYP19) in E. coli -- Expression of Recombinant Cytochromes c in E. coli -- Semi-Synthesis of Glycoproteins from E. coli through Native Chemical Ligation -- Expression of Recombinant Proteins with Uniform N-Termini -- Recent Developments in Difficult Protein Expression: A Guide to E. coli Strains, Promoters, and Relevant Host Mutations -- Periplasmic Chaperones Used to Enhance Functional Secretion of Proteins in E. coli -- Engineering Unusual Amino Acids into Peptides Using Lantibiotic Synthetase -- The Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membrane -- Detection of Protein-Protein Interactions in Bacteria by GFP-Fragment Reconstitution -- Enhancing the Solubility of Recombinant Proteins in Escherichia coli by Using Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner -- Introducing Predetermined Mutations throughout a Target Gene Using TDEM (Transposon Directed Base-Exchange Mutagenesis) -- Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase.
Keywords: Human genetics; Proteomics; Human Genetics; Proteomics