High-Resolution Imaging of Cellular Proteins : Methods and Protocols - Schwartzbach, Steven D.; Skalli, Omar.; Schikorski, Thomas; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture -- Antibody Production with Synthetic Peptides -- Production and Purification of Polyclonal Antibodies -- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2 and Streptavidin -- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications -- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy -- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells -- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses -- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells -- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging -- Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins -- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas -- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues -- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae -- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes -- Pre-Embedding Nanogold Silver and Gold Intensification -- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval -- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections -- Immuno-Gold Labelling for Scanning Electron Microscopy -- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope -- Functional Nanoscale Imaging Of Synaptic Vesicle Cycling With Superfast Fixation.
Keywords: Radiology; Imaging / Radiology