Metagenomics : Methods and Protocols - Streit, Wolfgang R.; Daniel, Rolf.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Construction of Small-Insert and Large-Insert Metagenomic Libraries -- Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies -- Construction and Screening Marine Metagenomic Large Insert Libraries -- Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment -- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms -- Assessing Bacterial and Fungal Diversity in the Plant's Endosphere -- Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities -- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production -- Degradation Network Reconstruction Guided by Metagenomic Data -- Novel Tools for the Functional Expression of Metagenomics DNA -- A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries -- Screening for Cellulase Encoding Clones in Metagenomics Libraries -- Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes -- Screening Glycosyltransferases for Polyphenol Modifications -- Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial Communities -- Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity -- Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes -- Screening for N-AHSL-Based-Signaling Interfering Enzymes -- Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites.
Keywords: Human genetics; Human Genetics