Host-Fungus Interactions : Methods and Protocols - Brand, Alexandra C.; MacCallum, Donna M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy -- Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans -- Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans -- Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi -- Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers -- Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System -- Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast -- Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach -- Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers -- RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum -- RNA Interference in Cryptococcus neoformans -- Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA -- Tetracycline-Inducible Gene Expression in Candida albicans -- Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii -- Modular Gene Over-Expression Strategies for Candida albicans -- Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans -- Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans -- Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils -- Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions -- Simple Assays for Measuring Innate Interactions with Fungi -- Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells -- Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium -- Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR -- In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus -- Biofilm Formation Studies in Microtiter Plate Format -- Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of Infection -- Laser Capture Microdissection of Candida albicans from Host Tissue -- Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples -- Cytokine Measurement Using Cytometric Bead Arrays (CBA) -- Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis -- Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi -- Drosophila melanogaster as a Model Organism for Invasive Aspergillosis -- Galleria mellonella as a Model for Fungal Pathogenicity Testing -- Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi -- Mouse Intravenous Challenge Models and Applications -- A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis -- Invasive Models of Histoplasmosis -- Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization -- A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections -- Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter -- Orogastrointestinal Model of Mucosal and Disseminated Candidiasis -- A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis.
Keywords: Microbiology; Pathology; Laboratory medicine; Microbiology; Pathology; Laboratory Medicine