Human Retrovirus Protocols : Virology and Molecular Biology - Zhu, Tuofu; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2005)
Methods for Virus Isolation and Detection -- Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals -- Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals -- Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes -- Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid -- Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue -- Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids -- Isolation and Quantification of HIV From Lymph Nodes -- Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes -- Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells -- Isolation of Foamy Viruses From Peripheral Blood Lymphocytes -- Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA -- Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR -- Detection of HIV-1 Provirus and RNA by In Situ Amplification -- Detection of HTLV-1 Gene on Cytologic Smear Slides -- Detection of HIV-2 by PCR -- Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions -- Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR -- Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR -- A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay -- A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity -- Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay -- Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization -- Detection of Drug-Resistant HIV-1 Strains -- Molecular Biology Methods -- Determination of Cell Tropism of HIV-1 -- Determination of Co-Receptor Usage of HIV-1 -- Quantitative Evaluation of HIV and SIV Co-Receptor Use With GHOST(3) Cell Assay -- Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals -- Methods to Determine HIV-1 Ex Vivo Fitness -- A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes -- PCR Amplification, Cloning, and Construction of HIV-1 Infectious Molecular Clones From Virtually Full-Length HIV-1 Genomes -- Amplification and Cloning of Near Full-Length HIV-2 Genomes -- Growth and Manipulation of a Human T-Cell Leukemia Virus Type 2 Full-Length Molecular Clone -- Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones -- Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumaretrovirus -- Assessing the Relative Efficacy of Antiretroviral Activity of Different Drugs on Macrophages -- Gene Expression Profiling of HIV-1 Infection Using cDNA Microarrays -- Determination of DC-SIGN and DC-SIGNR Repeat Region Variations.
Keywords: Microbiology; Microbiology