Hematopoietic Stem Cell Protocols - Bunting, Kevin D.; Qu, Cheng-Kui.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2014)
The Hematopoietic Stem Cell Landscape -- Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow -- Single-Cell PCR Profiling of Gene Expression in Hematopoiesis -- Hematopoietic Stem and Progenitor Cell Mobilization in Mice -- Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells -- Flow Cytometry Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells -- Gene Expression Profiling of Hematopoietic Stem Cells (HSC) -- Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells -- DNA Methylation Profiling of Hematopoietic Stem Cells -- Metabolic Characterization of Hematopoietic Stem Cells -- Nano Proteomic Assays on Hematopoietic Stem Cells -- Hematopoietic Differentiation of Pluripotent Stem Cells in Culture -- Ex vivo Assays to Study Self-Renewal, Long-Term Expansion and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells -- Ex vivo Expansion of Murine and Human Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviral Barcoding of Hematopoietic Stem Cells -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells.
Keywords: Cell biology; Stem cells; Cell Biology; Stem Cells