Legionella : Methods and Protocols - Buchrieser, Carmen; Hilbi, Hubert; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
Identification of Legionella in the Environment -- Identification of Legionella in Clinical Samples -- Sequence-Based Identification of Legionella -- Investigation of Outbreaks - Epidemiology -- Outbreak Investigations and Identification of Legionella in Contaminated Water -- Typing Methods for Legionella -- Culture, Media and Handling of Legionella -- The Intracellular Metabolism of Legionella by Isotopologue Profiling -- Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction -- Constructing Unmarked Gene Deletions in Legionella pneumophila -- Natural Biofilm Formation with Legionella pneumophila -- Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium -- Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila -- Analysis of Legionella Infection by Flow Cytometry -- Analysis of Legionella Infection Using RNAi in Drosophila Cells -- Immunofluorescence Imaging of Legionella Infection In vitro -- Electron Microscopy of Legionella and Legionella-Infected Cells -- Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages -- Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System -- Subcellular Localization of Legionella Dot/Icm Effectors -- Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase -- Legionella Phospholipases Implicated in Infection - Determination of Enzymatic Activities -- Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids -- Characterization of Legionella lipopolysaccharide -- The Tetrahymena and Acanthamoeba Model Systems -- Dictyostelium Host Response to Legionella infection: Strategies and Assays -- The Caenorhabditis elegans Model of Legionella Infection -- Infection of Nonphagocytic Host Cells by Legionella -- Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages -- The Mouse as a Model for Pulmonary Legionella Infection -- Assessment of Legionella-Specific Immunity in Mice -- The Guinea Pig Model of Legionnaires' Disease -- Human Susceptibility to Legionnaires´ Disease -- cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila -- Methods to Study Legionella Transcriptome in vitro and in vivo -- Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction -- Identification of Legionella Effectors Using Bioinformatic Approaches.
Keywords: Infectious diseases; Environmental health; Bacteriology; Infectious Diseases; Water and Health; Bacteriology