Human Embryonic Stem Cell Protocols - Turksen, Kursad; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts -- Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel -- Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix -- Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate -- Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells -- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR -- Study of Gap Junctions in Human Embryonic Stem Cells -- Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions -- Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells -- Genetic Manipulation of Human Embryonic Stem Cells -- Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System -- Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells -- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation -- Derivation of Endothelial Cells and Pericytes from Human Pluripotent Stem Cells -- Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts -- Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosa -- Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections -- Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering -- Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells -- Directed Differentiation of Human Embryonic Stem Cells into Neural Progenitors -- Direct Conversion of Pluripotent Human Embryonic Stem Cells Under Defined Culture Conditions into Human Neuronal or Cardiomyocyte Cell Therapy Derivatives -- Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small Molecule Inhibitor of Src Family Kinases -- Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells -- Accelerated Three-Dimensional Neuroepithelium Formation from Human Embryonic Stem Cells and Its Use for Quantitative Differentiation to Human Retinal Pigment Epithelium -- Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells -- Generation of Megakaryocytes and Platelets from Human Pluripotent Stem Cells -- A Simple Protocol for the Generation of Cardiomyocytes from Human Pluripotent Stem Cells.
Keywords: Cell biology; Cell Biology