Histones : Methods and Protocols - Guillemette, Benoit; Gaudreau, Luc R.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
In Vitro Assembly of Nucleosomes For Binding/Remodeling Assays -- An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro -- Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks -- Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays -- Histone Purification from Saccharomyces Cerevisiae -- Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid Extracted, Hplc-Purified Histones -- Silac-Based Quantitative Strategies for Accurate Histone Post-Translational Modifications Profiling Across Multiple Biological Samples -- Characterization of Individual Histone Post-Translational Modifications and their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies -- Production and Purification of Antibodies against Histone Modifications -- Immunofluorescence of Histone Proteins -- Acid-Urea Gel Electrophoresis and Western Blotting Of Histones -- Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast -- A Spiking Strategy for Chip-Chip Data Normalization in S. Cerevisiae -- High-Resolution Genome-Wide Mapping Of Nucleosome Positioning And Occupancy Level Using Paired-End Sequencing Technology -- Physarum Polycephalum for Studying the Function Of Histone Modifications In Vivo -- A Method for Large Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins. .
Keywords: Cell biology; Cell Biology