Microglia : Methods and Protocols - Joseph, Bertrand; Venero, José Luis.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
A Brief Overview of Multi-Talented Microglia -- Cell Culturing of Human and Murine Microglia Cell Lines -- Microglia Isolation from Adult Mouse Brain -- Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains -- Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology -- Isolation and Culture of Adult Human Microglia within Mixed Glial Cultures for Functional Experimentation and High Content Analysis -- Depletion of Microglia from Primary Cellular Cultures -- Lentiviral Transduction of Cultured Microglia -- Microglial Activation: Measurement of Cytokines by Flow Cytometry -- In situ Hybridization of Cytokine mRNA using Alkaline Phosphatase Labelled Oligodeoxynucleotide Probes -- Use of Meso-scale DiscoveryTM  to Examine Cytokinecontentin Microglia Cell Supernatant -- Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species -- Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells -- Quantification of Microglial Phagocytosis by a Flow Cytometer-based Assay -- Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry -- Fluorescence Imaging of Intracellular Ca2+, Na+ and H+ in Cultured Microglia -- Patch Clamp Protocols to Study Ion Channel Activity in Microglia -- Studying M1 and M2 States in Adult Microglia -- Isolating, Culturing, and Polarizing Primary Human Adult and Fetal Microglia -- Understanding Microglia-neuron Cross-talk: Relevance of the Microglia-neuron Co-cultures -- Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia -- Microglia Detection by Enzymatic Histochemistry -- Tomato Lectin Histochemistry for Microglial Visulization -- Immunohistochemical Detection of Microglia -- Intrathecal Infusion of Microglia Cells -- Intracranial Injection of LPS in Rat as Animal Model of Neuroinflammation -- Analyses of Microglia Effector Function Using CX3CR1-GFP Knock In Mice -- In vivo Two-photon Microscopy of Microglia -- Use of Confocal Microscopy in the Study of Microglia in a Brain Metastasis Model.
Keywords: Neurosciences; Neurobiology; Neurosciences; Neurobiology