In Situ Detection of DNA Damage : Methods and Protocols - Didenko, Vladimir V.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2002)
Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay) -- Labeling DNA Damage with Terminal Transferase -- TUNEL Assay -- Electron Microscopic Detection of DNA Damage Labeled by TUNEL -- Quantitative Differentiation of Both Free 3? OH and 5? OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy -- Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques -- In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry -- Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment -- DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment -- Labeling DNA Breaks In Situ by Klenow Enzyme -- In Situ Nick Translation at the Electron Microscopic Level -- Labeling DNA Breaks Using Ligase -- In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections -- Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase -- In Situ Detection of Double-Strand DNA Breaks with Terminal 5?OH Groups -- Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques -- The Comet Assay -- The Comet Assay -- Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays -- Application of FISH to Detect DNA Damage -- Detection of Modified Bases and AP Sites in DNA -- Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2?-Deoxyguanosine (8-OHdG) -- The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini -- Indirect and General Markers of DNA Damage -- Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage -- Ultrasound Imaging of Apoptosis -- p53 Induction as an Indicator of DNA Damage -- Detection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA).
Keywords: Human genetics; Cytology; Human Genetics; Cell Biology