Handbook of ELISPOT : Methods and Protocols - Kalyuzhny, Alexander E.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Unique Strengths of ELISPOT for T Cell Diagnostics -- The Impact of Harmonization on ELISPOT Assay Performance -- Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells -- Utilization of Feline ELISPOT for Mapping Vaccine Epitopes -- Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay -- Dual and Triple Color Fluorospot -- ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes -- ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells -- ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion -- Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell -- How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity -- Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay -- Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles -- Statistical Analysis of ELISPOT Assays -- Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories -- Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT -- ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis -- IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB® -- Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays.
Keywords: Immunology; Medical laboratories; Immunology; Laboratory Medicine