Human Airway Inflammation : Sampling Techniques and Analytical Protocols - Rogers, Duncan F.; Donnelly, Louise E.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Airway Inflammation and Remodeling in Asthma -- Airway Inflammation and Remodeling in Asthma -- Sample Collection -- Biopsy Techniques -- Bronchoalveolar Lavage (BAL) -- Nasal Secretions and Exudations -- Sample Collection -- Induced Sputum -- Induced Sputum -- Measurement of Exhaled Nitric Oxide and Carbon Monoxide -- Measurement of Exhaled Hydrocarbons -- Breath Condensate as a Vehicle for Collection of Inflammatory Mediators, Especially Hydrogen Peroxide -- Cell Isolation and Culture -- Airway Epithelial Cells (Primaries vs Cell Lines) -- Isolation and Culture of Human Alveolar Type II Pneumocytes -- Pulmonary Artery Endothelial Cells -- Isolation and Culture of Human Airway Smooth Muscle Cells -- Isolation and Purification of Human Mast Cells and Basophils -- Neutrophils -- Macrophages -- Collection, Separation, and Activation of Human T Lymphocytes -- Eosinophils -- Gene Expression/Regulation -- Analysis of Gene Expression -- Analysis of Gene Expression -- The Technique of In Situ Hybridization -- Measurement of Airway Mucin Gene Expression -- Transcription Factors and Inflammatory Gene Regulation -- Mediators -- Inflammatory Mediators in Spontaneously Produced Sputum -- Flow Cytometry -- Measurement of Granulocyte Pharmacodynamics in Whole Blood by Flow Cytometry -- Current In Vitro Models of Leukocyte Migration -- Tracing Intracellular Mediator Storage and Mobilization in Eosinophils -- Measurement of Metalloproteinases -- Immunocytochemistry and Immunohistochemistry -- The Measurement of Cysteinyl Leukotrienes in Urine -- Quantitative Analysis of Cyclooxygenase Metabolites of Arachidonic Acid -- Quantitative Analysis of F2 Isoprostanes.
Keywords: Pneumology; Pneumology/Respiratory System