HDAC/HAT Function Assessment and Inhibitor Development : Methods and Protocols - Krämer, Oliver H.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells -- Assessment of HDACi-Induced Protein Cleavage by Caspases -- Assessment of HDACi-Induced Cytotoxicity -- Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization -- Analysis of Histone Deacetylase-Dependent Effects on Cell Migration Using the Stripe Assay -- Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translation -- Effects of HDACi on Immunological Functions -- DNA Fiber Spreading Assay to Test HDACi Effects on DNA and Its Replication -- Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination -- Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research -- Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes Via Proximity Ligation Assay -- Purification and Analysis of Male Germ Cells from Adult Mouse Testis -- Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions -- Engineering of Conditional Class I Hdac Knockout Mice and Generation of a Time-Spatial Knockout by a Dual Recombination System -- Xenografting of Cancer Cell Lines for In Vivo Screening of the Therapeutic Potential of HDAC Inhibitors -- Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-Cancer Activities of HDACi -- Phospho-Flow Analysis of Primary Mouse Cells after HDAC Inhibitor Treatment -- Evaluation of Innate Immune Gene Expression Following HDAC Inhibitor Treatment by High Throughput qPCR and PhosFlow Cytometry -- Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP) -- Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases -- Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells -- Testing the Effects of SIAH Ubiquitin E3 Ligases on Lysine Acetyl Transferases -- Assessment of HDACi-Induced Acetylation of Non-Histone Proteins by Mass Spectrometry -- Assessing the Role of Paralog-Specific Sumoylation of HDAC1 -- Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques -- How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot -- Evaluating the Effect of HDAC8 Inhibition in Malignant Peripheral Nerve Sheath Tumors -- Analyzing the Impact of Pan- and Class-Specific HDACi on Differentiation-Associated Factors -- Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles -- Preparation of Histone Deacetylase Inhibitor Vorinostat Loaded Poly d, l-lactide-co-glycolide Polymeric Nanoparticles by Nanoprecipitation Method -- Generation and Assessment of Fusions Between HDACi and TKi.
Keywords: Proteins .; Protein Science