Hydrocarbon and Lipid Microbiology Protocols : Genetic, Genomic and System Analyses of Pure Cultures - McGenity, Terry J.; Timmis, Kenneth N.; Nogales, Balbina; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2017)
Introduction -- Broadening the SEVA plasmid repertoire to facilitate genomic editing of Gram-negative bacteria -- Protocols on regulation of gene expression -- Ultra-high-throughput transposon scanning of bacterial genomes -- Knock-in-leave-behind (KILB): Genetic grafting of protease-cleaving sequences into permissive sites of proteins with a Tn5-based transposition system -- Deep sequencing to study microbial transcriptomic responses to hydrocarbon degradation/production/stress -- Shotgun proteomics for hydrocarbon microbiology -- Interactomic characterization of membrane-associated mega-complexes for the anaerobic respiration in Pseudomonas aeruginosa -- Lipidomic analysis of bacteria by thin layer chromatography and liquid chromatography/mass spectrometry -- Accurate microbial genome annotation using an integrated and user-friendly environment for community expertise of gene functions: the MicroScope platform -- Approaches for displaying complete transcriptomes of environmental bacteria -- A practical protocol for integration of transcriptomics data into genome-scale metabolic reconstructions -- GC-MS based determination of mass isotopomer distributions for 13C-based metabolic flux analysis -- Analysis of the hierarchical and metabolic regulation of flux through metabolic pathways.
Keywords: Microbiology; Biochemical engineering; Microbiology; Biochemical Engineering