Listeria monocytogenes : Methods and Protocols - Jordan, Kieran; Fox, Edward M.; Wagner, Martin; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2014)
Sampling the Production/Processing Environment for Listeria -- Traditional Methods for Isolation of Listeria monocytogenes -- Confirmation of Isolates of Listeria by Conventional and Real-Time PCR -- Serotype Assignment by Sero-Agglutination, ELISA and PCR -- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes -- Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes -- Ribotyping and Automated Ribotyping of Listeria monocytogenes -- Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes -- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray -- Analysis of Listeria monocytogenes Subproteomes -- The Listeria Cell Wall and Associated Carbohydrate Polymers -- Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria -- Virulence Characterization of Listeria monocytogenes -- Internalization Assays for Listeria monocytogenes -- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes -- Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method -- Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes -- Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR -- Genome Sequencing of Listeria monocytogenes -- Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels -- Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers -- Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique -- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments.
Keywords: Microbiology; Food-Biotechnology.; Microbiology; Food Science