Monoclonal Antibodies : Methods and Protocols - Ossipow, Vincent; Fischer, Nicolas; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
Antigen Production for Monoclonal Antibody Generation -- Method for the Generation of Antibodies Specific for Site- and Post-translational Modifications -- Immunization, Hybridoma Generation and Selection for Monoclonal Antibody Production -- Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion -- Generation of Rabbit Monoclonal Antibodies -- Screening Hybridomas for Cell Surface Antigens by High-throughput Homogenous Assay and Flow Cytometry -- Screening and Subcloning of High Producer Transfectomas using Semi-solid Media and Automated Colony Picker -- Design and Generation of Synthetic Antibody Libraries for Phage Display -- Selection and Screening using Antibody Phage Display Libraries -- Yeast Surface Display for Antibody Isolation: Library Construction, Library Screening and Affinity Maturation -- Human B Cell Immortalization for Monoclonal Antibody Production -- Using Next-generation Sequencing for Discovery of High-frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice -- Cloning, Reformatting and Small Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat or Hamster Hybridoma -- Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid -- Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography -- Rapid Purification of Monoclonal Antibodies using Magnetic Microspheres -- Generation of Cell Lines for Monoclonal Antibody Production -- Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins -- Purification of Antibodies and Antibody Fragments using Capture Select Affinity Resins -- Reformatting of scFv antibodies into the scFv-Fc format and their Downstream Purification -- Antibody V and C Domain Sequence, Structure and Interaction Analysis with Special Reference to IMGT® -- Measuring Antibody Affinities as well as the Active Concentration of Antigens Present on a Cell Surface -- Determination of Antibody Structures -- Affinity Maturation of Monoclonal Antibodies by Multi Site-Directed Mutagenesis -- Epitope Mapping with Membrane-bound Synthetic Overlapping Peptides -- Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange and Peptide-Panning Techniques Combined with in silico Analysis -- Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen -- Epitope Mapping with Random Phage Display Library -- Epitope Mapping of Monoclonal and Polyclonal Antibodies using Bacterial Cell Surface Display -- Ion Exchange High Performance Liquid Chromatography (IEX-HPLC) -- Size Exclusion High Performance Liquid Chromatography (SEC-HPLC) -- N-glycosylation Characterization by Liquid Chromatography with Mass Spectrometry -- Fc-Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and their Functional Characterization -- Labeling and use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens -- Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization -- Antibody Array Generation and Use.
Keywords: Immunology; Laboratory medicine; Antibodies; Immunology; Laboratory Medicine; Antibodies