MicroRNA Expression Detection Methods - Wang, Zhiguo; Yang, Baofeng; SpringerLink (Online service) (Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2010)
General Remarks -- Detection, Profiling, and Quantification of miRNA Expression -- miRNA Microarray Methods -- Microarray and Its Variants for miRNA Profiling -- Northern Blotting Methods -- Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs -- In Situ Hybridization Methods -- In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs -- Real-Time RT-PCR Methods -- End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification -- miR-Q RT-PCR for miRNA Quantification -- Poly(A)-Tailed Universal Reverse Transcription -- Multiplexing RT-PCR for High-Throughput miRNA Profiling -- miRNA Amplification Profiling (mRAP) -- Cloning Methods -- miRNA Serial Analysis of Gene Expression (miRAGE or SAGE) -- Nanoparticle Methods -- Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis -- Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling -- Conducting Polymer Nanowires Technique for High-Sensitivity miRNA Expression Analysis -- Gold Nanoparticle Probe Method for miRNA Quantification -- Other Methods -- Splinted Ligation Method -- Padlock-Probes and Rolling-Circle Amplification -- Invader Assay -- Single Molecule Method -- Enzymatic Method -- Surface-Enhanced Raman Spectroscopy Method -- RAKE Assay -- Bead-Based Flow Cytometric miRNA Expression Profiling -- Bioluminescence miRNA Detection Method -- Molecular Beacon Method -- Ribozyme Method -- Electrocatalytic Moiety Labeling Technique for High-Sensitivity miRNA Expression Analysis -- Circulating miRNA Detection Methods -- Serum and Plasma miRNA Detection -- miRNA Detection from Peripheral Blood Microvesicles -- Detection of Placental miRNAs in Maternal Plasma -- Single-cell miRNA Detection Methods -- Quantitative LNA-ELF-FISH Method for miRNA Detection in Single Mammalian Cell -- Single Cell Stem-Looped Real-Time PCR -- miRNA Function-Reporter Expression Assay -- Whole Mount In Situ Analysis -- Whole Mount In Situ Hybridization (WM-ISH) for miRNA Expression Profiling During Vertebrate Development.
Keywords: Cell biology; Biochemistry; Gene expression; Pharmacology; Gene therapy; Cell Biology; Biochemistry, general; Gene Expression; Pharmacology/Toxicology.; Gene Therapy