Human Papillomaviruses : Methods and Protocols - Davy, Clare; Doorbar, John.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
Identification of New Papillomavirus Types -- Identification of HPV Variants -- Histochemical Analysis of Cutaneous HPV-Associated Lesions -- Histological Analysis of Cervical Intraepithelial Neoplasia -- Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections -- Detection and Quantitation of HPV Gene Expression Using Real-Time PCR -- Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers -- Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN -- HPV DNA Detection and Typing in Cervical Scrapes -- HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions -- Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies -- Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic "Raft" Cultures -- Differentiation of HPV-Containing Cells Using Organotypic "Raft" Culture or Methylcellulose -- Propagation of Infectious, High-Risk HPV in Organotypic "Raft" Culture -- Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic "Raft" Cultures -- The HPV Xenograft Severe Combined Immunodeficiency Mouse Model -- The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease -- Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology -- Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae -- Analysis of the Regulation of Viral Transcription -- Analysis of HPV Transcription by RPA -- Analysis of Regulatory Motifs Within HPV Transcripts -- Detection of HPV Transcripts by Nested RT-PCR -- Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays -- Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-time PCR -- Analysis of E7/Rb Associations -- Transformation Assays for HPV Oncoproteins -- Analysis of Adeno-Associated Virus and HPV Interaction -- In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins -- Measuring the Induction or Inhibition of Apoptosis by HPV Proteins -- Codon Optimization of Papillomavirus Genes -- Generation of HPV Pseudovirions Using Transfection and Their Use in Neutralization Assays -- Generation and Applications of HPV Pseudovirions Using Vaccinia Virus.
Keywords: Medical microbiology; Infectious diseases; Medical Microbiology; Infectious Diseases