Handbook of ELISPOT : Methods and Protocols - Kalyuzhny, Alexander E.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Challenges in Developing Protein Secretion Assays at a Single-Cell Level -- To Count of Not to Count: ELISPOT Evaluation Revisited -- Essential Controls for ELISpot Assay -- Automatic Search of Spots and Color Classification in ELISPOT Assay -- Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages -- Detection of Cross-Reactive B Cells Using the FluoroSpot Assay -- Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations -- Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays -- Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm -- Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite -- B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells -- Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT -- ELISPOT-Based "Multi-Color FluoroSpot" to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections -- Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses -- Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses -- Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection -- Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins -- Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines -- Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay.
Keywords: Immunology; Immunology