Immunocytochemical Methods and Protocols - Oliver, Constance; Jamur, Maria Célia.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Antibody Preparation -- Overview of Antibodies for Immunochemistry -- to the Purification of Antibodies -- Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration -- Antibody Purification: Ion-Exchange Chromatography -- Antibody Purification: Affinity Chromatography - Protein A and Protein G Sepharose -- Conjugation of Fluorochromes to Antibodies -- Biotinylation of Antibodies -- Tissue Preparation for Light Microscopic Analysis -- Cell Fixatives for Immunostaining -- Permeabilization of Cell Membranes -- Preparation of Frozen Sections for Analysis -- Processing of Cytological Specimens -- Processing of Tissue Culture Cells -- Processing of Tissue Specimens -- Heat-Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections -- Bright Field Detection Systems -- Fluorochromes: Properties and Characteristics -- Direct Immunofluorescent Labeling of Cells -- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts -- Overview of Conventional Fluorescence Photomicrography -- Overview of Confocal Microscopy -- Overview of Laser Microbeam Applications as Related to Antibody Targeting -- Immuno-Laser Capture Microdissection of Rat Brain Neurons for Real Time Quantitative PCR -- Overview of Antigen Detection Through Enzymatic Activity -- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods -- The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods -- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue -- Multiple Antigen Immunostaining Procedures -- Immunoenzymatic Quantitative Analysis of Antigens Expressed on the Cell Surface (Cell-ELISA) -- Use of Immunogold with Silver Enhancement -- Fluorescence-Activated Cell Sorter (FACS) Analyses -- Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry -- Tissue Disaggregation -- Indirect Immunofluorescent Labeling of Viable Cells -- Indirect Immunofluorescent Labeling of Fixed Cells -- Fluorescent Labeling of DNA -- Deparaffinization and Processing of Pathologic Material -- Colloidal Gold Detection Systems for Electron Microscopic Analysis -- Fixation and Embedding -- Preparation of Colloidal Gold -- Conjugation of Colloidal Gold to Proteins -- Colloidal Gold/Streptavidin Methods -- Pre-embedding Labeling Methods -- Postembedding Labeling Methods -- The Clinical Laboratory -- The Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines.
Keywords: Immunology; Pathology; Immunology; Pathology