Hepatitis C : Methods and Protocols - Tang, Hengli; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Detection and Quantification of HCV -- Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays -- Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen -- Immunohistochemical Detection of HCV Proteins in Liver Tissue -- HCV Nomenclature, Genotyping, and Quasispecies -- Nomenclature and Numbering of the Hepatitis C Virus -- A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates -- Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry -- HCV Replication in Vitro -- Purification and Crystallization of NS5A Domain I of Hepatitis C Virus -- NS5A Phosphorylation and Hyperphosphorylation -- Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies -- Structural and Functional Analysis of the HCV p7 Protein -- HCV Replicons: Overview and Basic Protocols -- Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA -- Studying HCV RNA Synthesis In Vitro with Replication Complexes -- Proteomics Study of the Hepatitis C Virus Replication Complex -- Investigation of the Hepatitis C Virus Replication Complex -- Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease -- Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay -- High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System -- Inhibition of HCV Replication by Small Interfering RNA -- HCV Entry and Infection -- Cellular Receptors and HCV Entry -- Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp) -- Screening of Small-Molecule Compounds as Inhibitors of HCV Entry -- Isolation of JFH-1 Strain and Development of an HCV Infection System -- Measuring HCV Infectivity Produced in Cell Culture and In Vivo -- Genotype 1a HCV (H77S) Infection System -- Full-Length Infectious HCV Chimeras -- Adaptation of the Hepatitis C Virus to Cell Culture -- Primary Human Hepatocyte Culture for HCV Study -- In Vivo Study of HCV in Mice with Chimeric Human Livers -- Immunity and Modeling -- Determination of HCV-Specific T-Cell Activity -- Analysis of HCV-Specific T Cells by Flow Cytometry -- Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp) -- Mathematical Modeling of HCV Infection and Treatment.
Keywords: Hepatology; Infectious diseases; Virology; Hepatology; Infectious Diseases; Virology