Herpes Simplex Virus : Methods and Protocols - Diefenbach, Russell J.; Fraefel, Cornel; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2014)
HSV-1 Biology and Life Cycle -- Herpes Simplex Virus Growth, Preparation, and Assay -- Isolation of Herpes Simplex Virus Nucleocapsid DNA -- Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes -- Engineering HSV-1 Vectors for Gene Therapy -- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors -- HSV-1 Amplicon Vectors as Genetic Vaccines -- Modification of HSV-1 to an Oncolytic Virus -- Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo -- Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals -- Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation -- Characterization of Extracellular HSV-1 Virions by Proteomics -- A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors -- Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions -- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy -- Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication -- Expression, Purification and Crystallization of HSV-1 Glycoproteins for Structure Determination -- Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components -- In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells -- HSV-1 Protein Expression Using Recombinant Baculoviruses -- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.
Keywords: Infectious diseases; Virology; Infectious Diseases; Virology