Molecular Toxicology Protocols - Keohavong, Phouthone; Grant, Stephen G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
Array-Based Immunoassays with Rolling-Circle Amplification Detection -- Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis -- Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage -- Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System -- Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques -- Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) -- Strategies for Measurement of Biotransformation Enzyme Gene Expression -- Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening -- TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material -- 32P-Postlabelling Analysis of DNA Adducts -- Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot -- DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry -- Analysis of DNA Strand Cleavage at Abasic Sites -- Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes -- Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk -- Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair -- Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice -- The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay -- Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice -- Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes -- Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems -- The Human T-Cell Cloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation -- Molecular Analysis of HPRT Mutations in Human Lymphocytes -- Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes -- Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis -- Detection of Point Mutations of K-ras Oncogene and p53 Tumor Suppressor Gene in Sputum Samples -- ACB-PCR Quantification of Somatic Oncomutations -- Gel-Based Nonradioactive Single Strand Conformational Polymorphism and Mutation Detection -- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesion -- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR -- Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells -- The Sister Chromatid Exchange (SCE) Assay -- The Gene Cluster Instability (GCI) Assay for Recombination -- Measuring Recombination Proficiency in Mouse Embryonic Stem Cells -- Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery -- Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic Nucleotide Excision Repair -- An Immunoassay for Measuring Repair of UV Photoproducts -- Analysis of Double-Strand Break Repair by Non-Homologous DNA End Joining in Cell-Free Extracts from Mammalian Cells -- Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF42 Extracellular Flux Analyzer and a Luciferase ATP Assay -- Quantification of Selective Phosphatidylserine Oxidation During Apoptosis -- Quantitative Method of Measuring Phophatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron -- Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.
Keywords: Pharmacology; Human genetics; Pharmacology/Toxicology.; Human Genetics