Hematopoietic Stem Cell Protocols - Bunting, Kevin D.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Overview -- The Expanding Tool Kit for Hematopoietic Stem Cell Research -- Stem Cell Enrichment and Analysis -- Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties -- Mobilization of Hematopoietic Stem and Progenitor Cells in Mice -- Transmigration of Human CD34+ Cells -- Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells -- Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry -- In Vitro Assays and Differentiation -- In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells -- Hematopoietic Development of Human Embryonic Stem Cells in Culture -- In Vitro Human T Cell Development Directed by Notch-Ligand Interactions -- In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C -- Transplantation Assays for Mouse and Human HSC -- Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection -- Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects -- Isolation and Functional Characterization of Side Population Stem Cells -- Transplantation of Chimeric Fetal Liver to Study Hematopoiesis -- Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair -- Genetic Modification of HSCs and Imaging Engraftment -- Retroviral Transduction of Murine Hematopoietic Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Stem Cells -- Retroviral Integration Site Analysis in Hematopoietic Stem Cells -- A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in Stem Cells -- Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models.
Keywords: Biochemistry; Cell biology; Hematology; Molecular biology; Biochemistry, general; Cell Biology; Hematology; Molecular Medicine