Lipoproteins and Cardiovascular Disease : Methods and Protocols - Freeman, Lita A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
Cloning Full-Length Transcripts and Transcript Variants Using 5' and 3' RACE -- Monitoring Gene Expression: Quantitative Real-Time RT-PCR -- Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases -- Northern Analysis of Gene Expression -- Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions -- Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research -- Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research -- Strategies for Designing Transgenic DNA Constructs -- Purification of Plasmid and BAC Transgenic DNA Constructs -- Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production -- Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes -- Generation of General and Tissue-Specific Gene Knockout Mouse Models -- Adeno-Associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism -- Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology -- Chromatin Immunoprecipitation -- Measurement of Lecithin:Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate -- Native-Native 2D Gel Electrophoresis for HDL Subpopulation Analysis -- Western Blots.
Keywords: Proteins .; Cardiology; Protein Science; Cardiology