JAK-STAT Signalling : Methods and Protocols - Nicholson, Sandra E.; Nicola, Nicos A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
Analysis of Janus Tyrosine Kinase Phosphorylation and Activation -- Co-Immunoprecipitation Protocol to Investigate Cytokine Receptor Associated Proteins e.g. Janus Kinases or Other Associated Signaling Proteins -- In Vitro JAK Kinase Activity and Inhibition Assays -- Quantitative Analysis of JAK Binding using Isothermal Titraion Calorimetry and Surface Plasmon Resonance -- Determination of Protein Turnover Rates in the JAK/STAT Pathway using a Radioactive Pulse-Chase Approach -- Designing RNAi Screens to Identify JAK/STAT Pathway Components -- The Use of JAK-Specific Inhibitors as Chemical Biology Tools -- Methods for Detecting Mutations in the Human JAK2 Gene -- Detection of Activated STAT Proteins -- Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents -- Flow Cytometric Analysis of STAT Phosphorylation -- Acetylation of Endogenous STAT Proteins -- Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining -- Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging -- Characterization of STAT Self-Association by Analytical Ultracentrifugation -- Constitutively Active STAT5 Constructs -- Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR -- Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis -- In vitro Ubiquitination of Cytokine Signaling Components -- Production and Crystallization of Recombinant JAK Proteins -- Bacterial Expression, Purification and Crystallization of Tyrosine Phosphorylated STAT Proteins.
Keywords: Cell biology; Cytokines; Growth factors; Cell Biology; Cytokines and Growth Factors