Human Pluripotent Stem Cells : Methods and Protocols - Schwartz, Philip H.; Wesselschmidt, Robin L.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
The Stem Cell Laboratory: Design, Equipment, and Oversight -- Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research -- Embryonic Stem Cell Derivation from Human Embryos -- Derivation of Human Parthenogenetic Stem Cell Lines -- Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer -- Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction -- Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons -- Traditional Human Embryonic Stem Cell Culture -- Xeno-Free Culture of Human Pluripotent Stem Cells -- Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging -- GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications -- Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging -- Classical Cytogenetics: Karyotyping Techniques -- FISH Analysis of Human Pluripotent Stem Cells -- Immunocytochemical Analysis of Human Pluripotent Stem Cells -- Flow Cytometric Analysis of Human Pluripotent Stem Cells -- The Teratoma Assay: An In vivo Assessment of Pluripotency -- Detection of Copy Number Variation using SNP Genotyping -- Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq -- Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays -- Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells -- Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells -- Lentivirus-mediated Modification of Pluripotent Stem Cells -- Nucleofection of Human Embryonic Stem Cells -- Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells -- Gene Targeting in Human Pluripotent Stem Cells -- Episomal Transgene Expression in Pluripotent Stem Cells -- The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures -- Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells -- Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells -- Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells -- In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells -- Differentiation of Dendritic Cells from Human Embryonic Stem Cells.
Keywords: Cell biology; Stem cells; Cell culture; Cell Biology; Stem Cells; Cell Culture