Membrane Protein Structure Determination : Methods and Protocols - Lacapère, Jean-Jacques.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Membrane Protein Purification -- Characterization of Membrane Protein Preparations: Measurement of Detergent Content and Ligand Binding After Proteoliposomes Reconstitution -- Native Membrane Proteins vs. Yeast Recombinant: An Example: The Mitochondrial ADP/ATP Carrier -- Bacterial Overexpressed Membrane Proteins: An Example: The TSPO -- Insect Cell Versus Bacterial Overexpressed Membrane Proteins: An Example, the Human ABCG2 Transporter -- X-Ray Crystallography -- Crystallography of Membrane Proteins: From Crystallization to Structure -- Structural Approaches of the Mitochondrial Carrier Family -- What Can Be Learned About the Function of a Single Protein from Its Various X-Ray Structures: The Example of the Sarcoplasmic Calcium Pump -- Recent Progress in the Structure Determination of GPCRs, a Membrane Protein Family with High Potential as Pharmaceutical Targets -- Electron Microscopy -- Observation of Membrane Proteins In Situ: AQPcic, the Insect Aquaporin Example -- Two-Dimensional Crystallization of Integral Membrane Proteins for Electron Crystallography -- Structure Determination of Membrane Protein by Both Cryo-Electron Tomography and Single Particle Analysis -- Electron Microscope Tomography of Native Membranes -- From Electron Microscopy Maps to Atomic Structures Using Normal Mode-Based Fitting -- Nuclear Magnetic Resonance -- Determination of Membrane Protein Structures Using Solution and Solid-State NMR -- Membrane Protein Fragments Reveal Both Secondary and Tertiary Structure of Membrane Proteins -- What Can We Learn from a Small Regulatory Membrane Protein? -- Solution-State NMR Spectroscopy of Membrane Proteins in Detergent Micelles: Structure of the Klebsiella pneumoniae Outer Membrane Protein A, KpOmpA -- NMR Spectroscopy of Lipid Bilayers -- Molecular Modelling -- Critical Review of General Guidelines for Membrane Proteins Model Building and Analysis -- 3D Structural Models of Transmembrane Proteins -- Molecular Dynamics of Membrane Peptides and Proteins: Principles and Comparison to Experimental Data -- Membrane Protein Dynamics from Femtoseconds to Seconds -- The Family of G Protein-Coupled Receptors: An Example of Membrane Proteins.
Keywords: Proteins .; Cell membranes .; Protein Science; Membrane Biology