Molecular and Cell Biology Methods for Fungi - Sharon, Amir.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Fungal Transformation and Gene Knockout -- Protoplast Transformation of Filamentous Fungi -- Electroporation and Agrobacterium-Mediated Spore Transformation -- High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains -- Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi -- DelsGate: A Robust and Rapid Method for Gene Deletion -- Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression -- Detection and Quantification of Fungi -- Analysis of Fungal Gene Expression by Real Time Quantitative PCR -- Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridization -- Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software -- Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR -- Application of Laser Microdissection to Study Plant-Fungal Pathogen Interactions -- Multiplex Gene Expression Analysis by TRAC in Fungal Cultures -- Amplification of Fungal Genomes Using Multiple Displacement Amplification -- Microscopy and Protein Analysis -- Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungus Neurospora crassa -- Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species -- A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae -- A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence -- Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi -- Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa -- Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi -- Evaluation of Antifungal Susceptibility Using Flow Cytometry -- Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling -- Split-EGFP Screens for the Detection and Localisation of Protein-Protein Interactions in Living Yeast Cells.
Keywords: Cell biology; Biochemistry; Mycology; Microbiology; Cell Biology; Biochemistry, general; Mycology; Eukaryotic Microbiology