Human Retroviruses : Methods and Protocols - Vicenzi, Elisa; Poli, Guido; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
Virion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay -- CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α -- The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating -- The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells -- Imaging HIV-1 Nuclear Pre-Integration Complexes -- HIV-1 Reverse Transcription -- RNase H: Specificity, Mechanisms of Action, and Antiviral Target -- HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat -- HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export -- HIV-1 Accessory Proteins: Nef -- HIV-1 Accessory Proteins: VpR -- HIV-1 Accessory Proteins: Vpu and Vif -- SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid Cells -- Imaging of HIV Assembly and Release -- HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells -- Determination of HIV-1 Co-Receptor Usage -- The Macrophage and HIV: Basic Concepts and Methodologies -- HIV Infection of Dendritic Cells -- Histocultures (Tissue Explants) in Human Retrovirology -- Single Copy Quantification of HIV-1 in Clinical Samples -- Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells -- HIV-1 Based Lentiviral Vectors -- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone -- Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques -- Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR -- Quantitative Analysis of Human T-Lymphotropic  Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR).
Keywords: Immunology; Virology; Immunology; Virology