Plastids : Methods and Protocols - Maréchal, Eric.; SpringerLink (Online service) (New York, NY : Springer US : Imprint: Humana, 2018)
Primary Endosymbioses: Emergence of the Primary Chloroplast and the Chromatophore, Two Independent Events -- Complex Endosymbioses I: From Primary to Complex Plastids, Multiple Independent Events -- Complex Endosymbiosis II: The Non-Photosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism -- Diversity and Plasticity of Plastids in Land Plants -- The Main Functions of Plastids -- Plastid Transient and Stable Interactions with Other Cell Compartments -- Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy -- Purification of Chloroplasts and Chloroplast Subfractions: Envelope, Thylakoids, and Stroma - From Spinach, Pea, and Arabidopsis thaliana -- Isolation of Inner and Outer Membranes of the Chloroplast Envelope from Spinach and Pea -- Purification of Non-Green Plastids (Proplastids and Amyloplasts) from Angiosperms, and Isolation of Their Envelope Membranes -- In Vitro Protein Import into Isolated Chloroplasts -- Analysis of the MTL Super-Complex at Contact Sites between Mitochondria and Plastids -- Isolation of Plastid-Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum -- Isolating the Plasmodium falciparum Apicoplast using Magnetic Beads -- Extraction and Quantification of Lipids from Plant or Algae -- Quantitative Assessment of the Chloroplast Lipidome -- Determination of the DNA/RNA-Associated Sub-Proteome from Chloroplasts and Other Plastid Types -- Mapping Plastid Transcript Population by Circular Reverse Transcription-Polymerase Chain Reaction -- Bioinformatic Analysis of Chloroplast Gene Expression and RNA Post-Transcriptional Maturations using RNA Sequencing -- A Guide to the Chloroplast Transcriptome Analysis using RNA-Seq -- A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae -- Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants -- Genetic Analysis of Chloroplast Biogenesis, and Function and Mutant Collections -- Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum -- In Silico Tools for the Prediction of Protein Import into Secondary Plastids -- AT_CHLORO - The First Step When Looking for Information about Subplastidial Localization of Proteins.
Keywords: Plant science; Botany; Plant Sciences