Nanotechnology for Nucleic Acid Delivery : Methods and Protocols - Ogris, Manfred; Sami, Haider; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection -- Synthesis of Bioreducible Polycations with Controlled Topologies -- Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery -- Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates -- Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems -- Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids -- Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery -- Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation -- Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol -- Layer-By-Layer Assembled Nanoparticles for siRNA Delivery -- Layer-By-Layer Film Engineering for Sequential Gene Delivery -- Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles -- In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films -- Lyophilization of Synthetic Gene Carriers -- Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery -- Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles -- Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery -- Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity -- Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity -- Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS) -- Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining -- Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery -- Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo -- Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery -- Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation -- Lipopeptide Delivery of siRNA to the Central Nervous System.
Keywords: Human genetics; Nanotechnology; Biotechnology; Pharmacology; Human Genetics; Nanotechnology; Biotechnology; Pharmacology/Toxicology.