Protein Misfolding Diseases : Methods and Protocols - Gomes, Cláudio M.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation -- Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, α-Synuclein, and Prion Aggregates -- Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides -- Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis -- A Multipronged Method for Unveiling Subtle Structural-Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies -- High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1 -- Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases -- Assays for Light Chain Amyloidosis Formation and Cytotoxicity -- Monitoring Aggregate Clearance and Formation in Cell-Based Assays -- Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor -- Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation -- Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry -- Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells -- A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction -- Super-Resolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells -- Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry -- Methods to Screen Compounds against Mutant p53 Misfolding and Aggregation for Cancer Therapeutics -- Early Stage Discovery and Validation of Pharmacological Chaperones for the Correction of Protein Misfolding Diseases -- Constructing Kinetically Controlled Denaturation Isotherms of Folded Proteins Using Denaturant-Pulse Chaperonin Binding -- In Vitro Prion Amplification Methodology for Inhibitor Screening -- SolubiS: Optimizing Protein Solubility by Minimal Point Mutations.
Keywords: Proteins .; Protein Science