Protein Secretion : Methods and Protocols - Economou, Anastassios; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
The Extraordinary Diversity of Bacterial Protein Secretion Mechanisms -- In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing -- Membrane Insertion of Small Proteins -- Membrane Protein Insertion in E. coli -- Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition -- In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm -- Sorting of Bacterial Lipoproteins to the Outer Membrane by the Lol System -- Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex -- Reconstitution of the SecY Translocon in Nanodiscs -- In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase -- Characterization of Interactions Between Proteins Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy -- Analysis of Tat Targeting Function and Twin-Arginine Signal Peptide Activity in Escherichia coli -- Site-Specific Cross-Linking of In Vitro Synthesized E. coli Preproteins for Investigating Transmembrane Translocation Pathways -- Tracking the Secretion of Fluorescently Labeled Type III Effectors from Single Bacteria in Real Time -- Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE -- Using Hidden Markov Models to Discover New Protein Transport Machines -- Bioinformatics Predictions of Localization and Targeting -- The Chloroplast Protein Import Machinery: A Review -- Measurement of the Energetics of Protein Transport Across the Chloroplast Thylakoid Membrane -- In Vitro Dissection of Protein Translocation into the Mammalian Endoplasmic Reticulum -- In Vitro Reconstitution of the Selection, Ubiquitination, and Membrane Extraction of a Polytopic ERAD Substrate -- Studying the ArfGAP-Dependent Conformational Changes in SNAREs -- Studying Endoplasmic Reticulum Function In Vitro Using siRNA -- High-Quality Immunofluorescence of Cultured Cells -- Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochondria: In Vivo and In Vitro Studies -- Native Techniques for Analysis of Mitochondrial Protein Import.
Keywords: Enzymology; Biochemistry; Proteomics; Enzymology; Biochemistry, general; Proteomics