Peroxisome Proliferator-Activated Receptors (PPARs) : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Badr, Mostafa Z. (Editor), Youssef, Jihan A. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edition:1st ed. 2013.
Series:Methods in Molecular Biology, 952
Subjects:
Table of Contents:
  • PPARs: History and Advances
  • PPAR-Alpha Cloning, Expression and Characterization
  • PPARGC1A and PPARG Genotyping. Beginner's Guide to Genotyping with Unlabeled Probes
  • Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPARβ/d Overexpression
  • Specific knockdown of PPARd Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering
  • Dominant-Negative and Knockdown Approaches to Studying PPAR activity
  • Producing PPARgamma2 Knockdown in Mouse Liver
  • Adipose Tissue-Specific PPARg Gene Targeting
  • Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs
  • PPAR SUMOylation: Some Useful Experimental Tips
  • Analyzing Phosphorylation-Dependent Regulation of Subcellular Transcriptional Activity of Transcriptional Coactivator NT-PGC-1a
  • In vivo Studies of PPAR-Chromatin Interactions: Chromatin Immunoprecipitation for Single-Locus and Genomewide Analyses
  • FISH Analysis Using PPARg  Specific Probes for Detection of PAX8-PPARg Translocation in Follicular Thyroid Neoplasms
  • Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin-Embedded Tissue Sections
  • Determination of PPAR Expression by Western-Blot
  • Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators
  • Estimation of the PPARa Agonism of Fibrates by a Combined MM-Docking Approach
  • Combined Biophysical and Cell-based Approaches for the Assessment of Ligand Binding to PPARg
  • Exploring PPAR Modulation in Experimental Mice
  • Induction of Adipogenic Differentiation in Three-Dimensional Culture Model on a Novel Microfabricated Scaffold
  • Analyzing PPAa/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry
  • Synthesis, Mass Spectrometric Characterization, and Analysis of the PPARd Agonist GW1516 and its Major Human Metabolites - Targets in Sports Drug Testing
  • LC-MS-Based Method for the Qualitative and Quantitative Analysis of the Novel PPARg Agonist KR-62980
  • Behavioral Paradigms to Evaluate PPAR Modulation in Animal Models of Brain Injury.