NADPH Oxidases : Methods and Protocols - Knaus, Ulla G.; Leto, Thomas L.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Intersecting Stories of the Phagocyte NADPH Oxidase and Chronic Granulomatous Disease -- Mammalian NADPH Oxidases -- Enhanced Immunoaffinity Purification of Human Neutrophil Flavocytochrome B for Structure Determination by Electron Microscopy -- Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs) -- A Close-Up View of the Impact of Arachidonic Acid on the Phagocyte NADPH Oxidase -- NOX5 Cell-Free Assay for the High-Throughput Screening of Small Molecules -- Spectroscopy of NOX Protein Family Members -- Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases -- Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays -- The X-CGD PLB-985 Cell Model for NOX2 Structure-Function Analysis -- Functional Characterization of DUOX Enzymes in Reconstituted Cell Models -- Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR -- Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells -- HPLC-Based Monitoring of Oxidation of Hydroethidine for Detection of NADPH Oxidase-Derived Superoxide Radical Anion -- Visualization of Intracellular Hydrogen Peroxide with the Genetically-Encoded Fluorescent Probe HyPer in NIH-3T3 Cells -- Imaging Intracellular H2O2 with the Genetically-Encoded PerFRET and OxyFRET Probes -- Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae -- Kinetic Analysis of Phagosomal ROS Generation -- Imaging Intestinal ROS in Homeostatic Conditions Using L-012 -- Hydro-Cy3-Mediated Detection of Reactive Oxygen Species In Vitro and In Vivo -- Phosphorylation of Gp91phox/NOX2 in Human Neutrophils -- The Molecular Regulation and Functional Roles of NOX5 -- Using Synthetic Peptides for Exploring Protein-Protein Interactions in the Assembly of the NADPH Oxidase Complex -- Rational Design and Delivery of NOX-Inhibitory Peptides -- High-Throughput Screening of NOX Inhibitors -- Protein-Protein Interaction Assay to Analyze NOX4/p22phox Heterodimerization -- Isolation of Redox Active Endosomes (Redoxosomes) and Assessment of NOX Activity -- Model Systems to Investigate NOX-Dependent Cell Migration and Invasiveness -- NADPH Oxidases and Aging Models of Lung Fibrosis -- Proteomic Methods to Evaluate NOX-Mediated Redox Signaling -- Neutrophil Extracellular Traps -- Chronic Granulomatous Disease -- Diagnostic Testing for Chronic Granulomatous Disease -- Gastrointestinal Complications in Chronic Granulomatous Disease -- Ex Vivo Models of Chronic Granulomatous Disease -- Gene Editing in Chronic Granulomatous Disease -- DUOX Defects and Their Roles in Congenital Hypothyroidism -- NAPDH Oxidases in Inflammatory Bowel Disease.
Keywords: Enzymology; Human physiology; Human genetics; Enzymology; Human Physiology; Human Genetics