Programmed Necrosis : Methods and Protocols - Ting, Adrian T.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Tools in the Art of Studying Necroptosis -- Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules -- Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis -- Distinguishing Necroptosis from Apoptosis -- Methods for Studying TNF-Mediated Necroptosis in Cultured Cells -- Analysis of Necroptosis in Bone Marrow-Derived Macrophages -- Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis -- Detection of MLKL Oligomerization during Programmed Necrosis -- Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation -- Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis -- Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis -- Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock -- Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury -- Assessment of In Vivo Kidney Cell Death: Glomerular Injury -- Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling -- Characterization of the TNFR1-SC using 'Modified Tandem Affinity Purification' in Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS) -- Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex -- Analysis of CYLD Proteolysis by Caspase-8 in Bone Marrow-Derived Macrophages.
Keywords: Cell biology; Cell Biology