Protein Complex Assembly : Methods and Protocols - Marsh, Joseph A.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Experimental Characterisation Of Protein Complex Structure, Dynamics, and Assembly -- High-Throughput Electron Cryo-Tomography of Protein Complexes and their Assembly -- Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single Particle Electron Microscopy -- Time-resolved Cryo-Electron Microscopy Using a Microfluidic Chip -- Characterizing Protein-protein Interactions using Solution NMR Spectroscopy -- Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit For Solution-state and Solid-state NMR -- Characterizing Protein-Protein Interactions using Deep Sequencing Coupled to Yeast Surface Display -- Structurally Guided In Vivo Crosslinking -- Characterising Intact Macromolecular Complexes using Native Mass Spectrometry -- Hydrogen Deuterium Exchange Mass Spectrometry to Study Protein Complexes -- Structural Analysis of Protein Complexes by Cross-linking and Mass-spectrometry -- Global Characterization of Protein Complexes by Biochemical Purification-mass Spectrometry (BP/MS) -- Proteomic Profiling of Integrin Adhesion Complex Assembly -- Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies -- Correlative 3D Structured Illumination Microscopy and Single Molecular Localisation Microscopy for Imaging Cancer Invasion -- Observing the Assembly of Protein Complexes In Living Eukaryotic Cells In Super-resolution using refSOFI -- Detecting Purinosome Metabolon Formation with Fluorescence Microscopy -- Analysis of Bacterial pilus Assembly by Shearing and Immunofluorescence Microscopy -- Expression, Purification and Assembly of Archaellum Subcomplexes of Sulfolobus Acidocaldarius -- Unstable Protein Purification through the Formation of Stable Complexes -- Expressing Multi-subunit Complexes using biGBac -- Computational Modelling of Protein Complex Structure and Assembly -- Inferring and using Protein Quaternary Structure Information from Crystallographic -- Searching and Extracting Data from the EMBL-EBI Complex Portal -- Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-Purification Data -- A Multi-scale Computational Model for Simulating the Kinetics of Protein Complex Assembly -- Flexible Protein-protein Docking with SwarmDock -- Protein-protein Docking using Evolutionary Information -- Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles -- Modeling the Structure of Helical Assemblies with Experimental Constraints in -- Selecting Conformational Ensembles Using Residual Electron and Anomalous Density (READ). .
Keywords: Proteins .; Protein Science