Phytochromes : Methods and Protocols - Hiltbrunner, Andreas; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Investigation of Light-Regulated Protein-Protein Interactions using Yeast Two-Hybrid Assays -- Rapid Examination of Phytochrome-PHYTOCHROME INTERACTING FACTOR (PIF) Interaction by In Vitro Co-Immunoprecipitation Assay -- Characterization of Light-Regulated Protein-Protein Interactions by In Vivo Co-Immunoprecipitation (Co-IP) Assays from Plant -- Detection of Phytochrome Phosphorylation in Plants -- Detection of SUMOylated Phytochromes in Plants -- Approaches to Identify Protein Ubiquitination Sites in Plants -- Expression, Purification, and Spectral Characterization of Phytochromes -- In Vivo Spectroscopy -- Basic Phytochrome B Calculations -- Quantitative Analysis of Photobodies -- Regulation of Alternative Splicing by Phytochrome -- Phytochrome-Regulation of Seed Germination -- Shade Avoidance and Neighbor Detection -- A Simple Method for Quantification of Protochlorophyllide in Etiolated Arabidopsis Seedlings -- Measuring Phytochrome-Dependant Light-Input to the Plant Circadian Clock -- Characterization of Flowering Time Mutants -- Rapid Detection of Hormonal Involvement in Light Responses -- Phytochrome and Light Signaling in Marchantia -- Analysis of Physcomitrella Phytochrome Mutants via Photo- and Polarotropism -- CRISPR/Cas9-Mediated Knockout of Physcomitrella patens Phytochromes -- Phylogenetic Methods to Study Light Signaling.
Keywords: Plant science; Botany; Plant Sciences