Protein Expression in Mammalian Cells : Methods and Protocols - Hartley, James L.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Why Proteins in Mammalian Cells? -- Large Scale Transfection of Mammalian Cells -- Selection of High Expressing Mammalian Cells by Surface Display of Reporters -- Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles -- Transfection of Difficult-to-Transfect Primary Mammalian Cells -- Stable Protein Expression in Mammalian Cells Using Baculoviruses -- Using Matrix Attachment Regions to Improve Recombinant Protein Production -- Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells -- Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells -- Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells -- Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker -- Tools for Co-Producing Multiple Proteins in Mammalian Cells -- Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases -- Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA -- Methods for Constructing Clones for Protein Expression in Mammalian Cells -- Optimizing Transient Recombinant Protein Expression in Mammalian Cells.
Keywords: Proteins .; Protein Science