Plant-Pathogen Interactions : Methods and Protocols - Birch, Paul.; Jones, John T.; Bos, Jorunn I.B.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
Galaxy as a Platform for Identifying Candidate Pathogen Effectors -- Bioinformatic analysis of expression data to identify effector candidates -- Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes -- On The Statistics Of Identifying Candidate Pathogen Effectors -- High-throughput imaging of plant immune responses -- In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats -- Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors -- Purification of fungal haustoria from infected plant tissue by flow cytometry -- Functional Characterisation of Nematode Effectors in Plants -- Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana -- Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids -- A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana -- Simple quantification of in planta fungal biomass -- Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum -- DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants -- A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector - Host Protein Complexes -- An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors -- Production of RXLR effector proteins for structural analysis by X-ray crystallography -- The Do's and Don'ts of effectoromics -- Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice -- A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals -- Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery.
Keywords: Plant science; Botany; Plant genetics; Plant Sciences; Plant Genetics and Genomics