Natural Killer Cell Protocols : Cellular and Molecular Methods - Campbell, Kerry S.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils -- In Vitro Development of Human Killer-Immunoglobulin Receptor-Positive NK Cells -- Subset Analysis of Human and Mouse Mature NK Cells -- Assessing Licensing of NK Cells -- Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo -- Use of Transfected Drosophila S2 Cells to Study NK Cell Activation -- Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry -- Studying NK Cell/Dendritic Cell Interactions -- Analysis of the NK Cell Immunological Synapse -- Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry -- Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells -- A Model System for Studying NK Cell Receptor Signaling -- Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction -- Lentiviral Gene Transduction in Human and Mouse NK Cell Lines -- of shRNAs into Human NK-Like Cell Lines with Retrovirus -- of shRNAs into Primary NK Cells with Lentivirus -- Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands -- Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation -- Identification of NK Cell Receptor Ligands Using a Signaling Reporter System -- Determining Ligand Specificity of Ly49 Receptors -- Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis -- A Simple Method to Measure NK Cell Cytotoxicity In Vivo -- Functional Analysis of Human NK Cells by Flow Cytometry -- Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry -- KIR Genotyping by Multiplex PCR-SSP -- Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR?) Genes -- Use of Inbred Mouse Strains to Map Recognition Receptors of MCMV Infected Cells in the NK Cell Gene Locus -- Studying NK Cell Responses to Ectromelia Virus Infections in Mice -- Activation of Human NK Cells by Malaria-Infected Red Blood Cells -- Natural Killer Cells in Human Pregnancy -- Analysis of Uterine Natural Killer Cells in Mice -- Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria.
Keywords: Immunology; Cell culture; Immunology; Cell Culture