Notch Signaling : Methods and Protocols - Bellen, Hugo J.; Yamamoto, Shinya; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2014)
Introduction to Notch Signaling -- Genetic Screens to Identify New Notch Pathway Mutants in Drosophila -- Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes -- Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice -- Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster -- Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila -- Tracking Trafficking of Notch and its Ligands in Mammalian Cells -- Visualizing Notch Signaling in vivo in Drosophila Tissues -- Monitoring Notch Activity in the Mouse -- Notch Signaling Assays in Drosophila Cultured Cell Lines -- Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays -- Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays -- Visualization of Notch Signaling Oscillation in Cells and Tissues -- Proteomic Analysis of the Notch Interactome -- Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands -- Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry -- Assay to Probe Proteolytic Processing of Notch by γ-secretase -- Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay -- Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay -- Probing the Epigenetic Status at Notch Target Genes -- Notch-ligand Binding Assays in Drosophila Cells -- Modeling Notch Signaling: A Practical Tutorial -- Small Molecules that Inhibit Notch Signaling -- Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling -- Application of Anti-ligand Antibodies to Inhibit Notch Signaling.
Keywords: Cell biology; Cell Biology