Proteostasis : Methods and Protocols - Matthiesen, Rune.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
UPS Activation in the Battle against Aging and Aggregation-Related Diseases: An Extended Review -- Review And Literature Mining On Proteostasis Factors' And Cancer -- Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role Of Usp45 in the Retina -- Immunodepletion and Immunopurification as Approaches for CSN Research -- Studying Protein Ubiquitylation in Yeast -- Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein -- In Vitro Ubiquitination - Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays -- Isolation of the Ubiquitin-Proteome From Tumour Cell Lines and Primary Cells Using Tubes -- Tubes-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome -- Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples -- Method for the Purification of Endogenous Unanchored Polyubiquitin Chains -- Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System -- Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells -- Monitoring Ubiquitin-Coated Bacteria Via Confocal Microscopy -- Detection and Analysis of Cell Cycle-Associated Apc/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo -- Detection and Analysis Of Sumoylation Substrates In Vitro and In Vivo. - Detection of Protein-Protein Interactions and Post-Translational Modifications using the Proximity Ligation Assay: Application to the Study of the Sumo Pathway -- Dissecting of Sumo Dynamics by Mass Spectrometry -- Isolation of Lysosomes from Mammalian Tissues and Cultured Cells -- Analysis Of Relevant Parameters For Autophagic Flux Using Hela Cells Expressing Egfp-Lc3 -- Analysis of Protein Oligomeric Species by Sucrose Gradients -- Analysis of Protein Oligomerization by Electrophoresis.-Blot-Ms of Carbonylated Proteins: A Tool to Identify Oxidized Proteins -- Quantitation of Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry -- A Simple Protocol for High Efficiency Protein Isolation after RNA Isolation from Mouse Thyroid and Other Very Small Tissue Samples -- Monitoring Target Engagement Of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future -- Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues -- High-Throughput Sirna Screening Applied to the Ubiquitin-Proteasome System -- High-Throughput Yeast-Based Reporter Assay to Identify Compounds with Anti-Inflammatory Potential -- Using Alphascreen ® to Identify Small Molecule Inhibitors Targeting a Conserved Host-Pathogen Interaction -- Global MS-Based Proteomics Drug Profiling.
Keywords: Proteins .; Protein Science