RNA-Protein Complexes and Interactions : Methods and Protocols - Lin, Ren-Jang.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2 -- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification) -- Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro -- Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting -- Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture -- Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts -- In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly -- Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases -- Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles -- Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture -- Measuring mRNA Translation by Polysome Profiling -- Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq -- Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment -- Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks -- Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking -- Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX -- mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins -- Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors -- Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5' Splice-Site Base-Pairing -- Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter.
Keywords: Proteins .; Protein Science