Mycobacteria Protocols - Parish, Tanya; Roberts, David M.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly -- Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis -- RNA Sequencing for Transcript 5'-end Mapping in Mycobacteria -- Fractionation and Analysis of Mycobacterial Proteins -- Lipid and Lipoarabinomannan Isolation and Characterization -- Metabolomics of Mycobacterium tuberculosis -- Electroporation of Mycobacteria -- Targeted Gene Knockout and Essentiality Testing by Homologous Recombination -- Construction of Conditional Knockdown Mutants in Mycobacteria -- Mycobacterial Recombineering -- In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria -- Genetic Dissection of Mycobacterial Biofilms -- Measuring Efflux and Permeability in Mycobacteria -- Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy -- Antimicrobial Susceptibility Testing for Mycobacterium sp. -- Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis -- Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis -- A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis -- Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis -- Macrophage Infection Models for Mycobacterium tuberculosis -- Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis -- Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids -- Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum -- Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays.  .
Keywords: Microbiology; Microbiology